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Formalin Fixed Tissue

The Formalin Fixed Tissue sample needs to fixed so that all ongoing biochemical reactions cease and also potentially to increase the mechanical or structural strength or stability of the treated tissue.

The sample tissue is submerged in a 10% neutral buffered formalin, the most commonly used fixative in histology. This fixes tissues by cross-linking the proteins; primarily residues of the basic amino acid lysine. Its effects are reversible by excess water and it avoids formalin pigmentation. Formalin fixation also allows long-term storage, good tissue penetration, and it is optimal for immunohistochemistry techniques.

Formalin fixed tissue is the most prevalent form of fixation and creates an optimal condition in the sample tissue for encasing it in paraffin wax.

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