Formalin fixation helps to ensure that all ongoing biochemical reactions cease and can potentially increases the mechanical & structural strength and stability of the treated tissue.

The sample tissue is submerged in a 10% neutral buffered formalin, the most commonly used fixative in histology. This fixes tissues by cross-linking the proteins; primarily residues of the basic amino acid lysine. The effects are reversible by excess water and it avoids formalin pigmentation. Formalin fixation also allows long-term storage, good tissue penetration, and it is optimal for immunohistochemistry techniques.

Formalin fixation is the most prevalent form of tissue preservation and creates optimal tissue sample conditions for encasing samples in paraffin wax.

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